Lo mkhiqizo uyisibonakhulu esigxile ekhaleni esiklanyelwe i-patch clamp electrophysiology noma isayensi yezinto.Izitendi ze-manipulator gantry ezizinze kakhulu, ezilungisekayo zithatha indawo yozimele wesibonakhulu esivamile, okuvumela inqwaba yokulungiselelwa okulungisekayo kobude.Izinga le-epi lingahlonyiswa ngekhiyubhu yesihlungi esisodwa noma i-Olympus epi-illuminator ephelele.Isistimu yokukhanya okudluliswayo itholakala ngesibani esisodwa esimhlophe se-LED noma isibani esimhlophe esikabili kanye nama-IR LED.Ukukhanyisa okudlulisiwe kusebenzisa i-condenser ye-Olympus Oblique Coherent Contrast (OCC), noma izingxenye ze-IR-DIC ngezindlela ezitholakalayo zokuqhathanisa.
I(ama)LED ingacushwa ngesignali yedijithali.Lokhu kuqeda isidingo samashutha futhi kwengeza amandla okwenza izithombe zisuka endaweni ye-trans.Ezivivinyweni lapho ukukhanya okudlulisiwe kungafunwa, i-LED, i-condenser focus mechanism kanye ne-condensing optics zisuswa kalula njengenhlangano eyodwa.Ukwengeza, indlela yokukhanya edlulisiwe ifushane kunezinye izinhlelo, okuvumela umzimba we-microscope ukuthi uhlale ngaphansi kakhulu kune-microscope evamile.Isibonakhulu esifushane sihumusha ukuzinza okwengeziwe, ukwanda kwe-ergonomics, kanye nokusebenziseka kalula.
Isibonakhulu se-NAN singalungiselelwa ngezicucu zamehlo ezintathu ukuze zibonwe ngeso lengqondo, noma ngenye indlela, ngelensi yeshubhu kanye ne-C-mount uma kufunwa ikhamera kuphela.Ukuze uqedele i-electrophysiology “rig”, siphinde sinikeze ngohlu oluningi lwezinsiza, ezifaka ezinye imithombo yokukhanya ye-epi-fluorescence, izikhohlisi, namasistimu wokuhlanganisa ama-amplifier.
IZICELO
- I-Patch clamp electrophysiology
- in vivo, in vitro, futhi sika
- Ukuqoshwa kweseli lonke
- Ukuqoshwa kwe-intracellular
- Isayensi yezinto ezibonakalayo
IZICI
- Isiteji se-XY esizinzile esinemoto noma umhumushi onenjini
- Vula isibonakhulu somklamo ngokugxila kwemoto
- Ilungiseka ngokushesha ngokusekelwe ezidingweni zokuhlola
- Ilungiselelwe ukuvumela ukuhlolwa kwe-vivo kanye ne-vitro ekusetheni okukodwa
- Idizayinelwe ukusetshenziswa namalensi enhloso ye-Olympus
- Isofthiwe yamahhala ye-Multi-Link™ ihlanganisa ukunyakaza ngokuma kwe-micropipette
- I-Oblique Coherent Contrast (OCC) noma i-Differential Interference Contrast (DIC)
- Ukukhanya kwe-Epi-fluorescent
Isikhathi sokuthumela: Mar-15-2023